Inhibition of influenza virus infection by targeting genome conserved region with non-natural nucleic acid

Nucleic Acids Symp Ser (Oxf). 2009:(53):285-6. doi: 10.1093/nass/nrp143.

Abstract

Two highly conserved 15 base sequences of influenza A virus genome were identified by CONSERV software, which can detect contiguous conserved sequences of biological sequences. Antiviral effect of phosphorothioate oligonucleotide that target these conserved sequences was evaluated by plaque formation assay and cell viability assay. Pre-treatment of cells with anti-PB2 (RNA polymerase subunit) reduced the size of plaques in a sequence dependent manner. Post-treatment of cells with anti-PB2 phosphorothioate oligonucleotide inhibited the virus-induced cytotoxicity.

MeSH terms

  • Animals
  • Base Sequence
  • Cell Line
  • Conserved Sequence
  • Dogs
  • Genome, Viral
  • Influenza A virus / genetics*
  • Influenza A virus / growth & development
  • Phosphorothioate Oligonucleotides* / chemistry
  • RNA, Viral / chemistry
  • RNA-Dependent RNA Polymerase / antagonists & inhibitors
  • RNA-Dependent RNA Polymerase / genetics
  • Viral Plaque Assay
  • Viral Proteins / antagonists & inhibitors
  • Viral Proteins / genetics

Substances

  • PB2 protein, Influenzavirus A
  • Phosphorothioate Oligonucleotides
  • RNA, Viral
  • Viral Proteins
  • RNA-Dependent RNA Polymerase