Multipyrene tandem probes for detection of C677T polymorphism in MTHFR gene

Nucleic Acids Symp Ser (Oxf). 2009:(53):143-4. doi: 10.1093/nass/nrp072.

Abstract

We designed tandems of oligo(2'-O-methylribonucleotides) conjugates containing two bispyrene (5'-bisPyr and 3'-bisPyr) groups on their junction for detection of C677T polymorphism in the methylenetetrahydrofolate reductase gene (MHTFR). The potential of SNP detection with multipyrene tandems of oligo(2'-O-methylribonucleotides) was demonstrated.

MeSH terms

  • Fluorescent Dyes / chemistry*
  • Methylenetetrahydrofolate Reductase (NADPH2) / genetics*
  • Oligonucleotide Probes / chemistry*
  • Polymorphism, Single Nucleotide*
  • Pyrenes / chemistry

Substances

  • Fluorescent Dyes
  • Oligonucleotide Probes
  • Pyrenes
  • Methylenetetrahydrofolate Reductase (NADPH2)