Abstract
Using alternative enzymes for on-line digestion with a triaxial electrospray probe extends sequence coverage. This is the first report of utilization of our triaxial probe for on-line analysis with enzymes other than pepsin, suggesting potential for broader application. The probe allows access to processes occurring on a timescale and/or involving substrate conformations complementary to those for conventional (off-line) digestion. Some of the features observed in application to Abeta fibrils are suggestive of unique reactive intermediates during dissolution. Data obtained with enzyme mixtures suggest synergistic effects.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Amino Acid Sequence
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Amyloid beta-Peptides / chemistry*
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Animals
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Aspartic Acid Endopeptidases / chemistry*
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Hydrogen Bonding
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Hydrolysis
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Molecular Sequence Data
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Online Systems*
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Pepsin A / metabolism
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Peptide Fragments / chemistry*
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Peptide Hydrolases / chemistry*
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Peptide Hydrolases / classification
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Spectrometry, Mass, Electrospray Ionization / methods*
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Swine
Substances
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Amyloid beta-Peptides
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Peptide Fragments
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amyloid beta-protein (1-40)
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Peptide Hydrolases
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Aspartic Acid Endopeptidases
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Endothia aspartic proteinase
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Pepsin A