The evaluation of the possibilities of using PLGA co-polymer and its composites with carbon fibers or hydroxyapatite in the bone tissue regeneration process - in vitro and in vivo examinations

Magdalena Cieślik; Anna Mertas; Anna Morawska-Chochół; Daniel Sabat; Rajmund Orlicki; Aleksander Owczarek; Wojciech Król; Tadeusz Cieślik

doi:10.3390/ijms10073224

The evaluation of the possibilities of using PLGA co-polymer and its composites with carbon fibers or hydroxyapatite in the bone tissue regeneration process - in vitro and in vivo examinations

Int J Mol Sci. 2009 Jul 15;10(7):3224-3234. doi: 10.3390/ijms10073224.

Authors

Magdalena Cieślik¹, Anna Mertas², Anna Morawska-Chochół³, Daniel Sabat⁴, Rajmund Orlicki¹, Aleksander Owczarek⁵, Wojciech Król², Tadeusz Cieślik⁶

Affiliations

¹ Chair and Department of Stomatological Material Science, Pl. Akademicki 17, 41 907 Bytom, Medical University of Silesia, Katowice, Poland.
² Chair and Department of Microbiology and Immunology, Jordana 19, 41 808 Zabrze, Medical University of Silesia, Katowice, Poland.
³ Department of Biomaterials, Faculty of Materials Science and Ceramics, Al. Mickiewicza 30, 30 059 Cracow, AGH University of Science and Technology, Cracow, Poland.
⁴ Department and Institute of Pathomorphology, 3maja 13-15, 41 800 Zabrze, Medical University of Silesia, Poland.
⁵ Division of Statistics, Ostrogórska 30, 95 010 Sosnowiec, Medical University of Silesia, Katowice, Poland.
⁶ Department and Clinic of Maxillofacial Surgery, Francuska 20/24, 40 027 Katowice, Medical University of Silesia, Katowice, Poland.

Abstract

Synthetic polymers belonging to the aliphatic polyester group have become highly promising biomaterials for reconstructive medicine. The purpose of the present work is a biological evaluation of lactide-glycolide co-polymer (PLGA) and its composites with carbon fibers (PLGA+CF) or hydroxyapatite (PLGA+HA). The cytotoxicity of the evaluated materials towards hFOB 1.19 human osteoblast-like cells was assessed. Moreover, during the one-year contact of the assessed materials with living osseous tissue, the progress of bone formation was analyzed and the accompanying process of the materials' degradation was evaluated. The materials under evaluation proved to be biocompatible.

Keywords: PLGA co-polymer; biocompatibility; carbon fibers; composites; cytotoxicity; human osteoblast-like cells; hydroxyapatite; in vivo experiments on animals.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Biocompatible Materials / chemistry*
Biocompatible Materials / toxicity
Carbon / chemistry*
Carbon / toxicity
Carbon Fiber
Cell Survival
Cells, Cultured
Durapatite / chemistry*
Durapatite / toxicity
Humans
Lactic Acid / chemistry*
Lactic Acid / toxicity
Mandible / drug effects
Mandible / physiology
Osseointegration*
Osteoblasts / drug effects
Osteoblasts / metabolism
Polyglycolic Acid / chemistry*
Polyglycolic Acid / toxicity
Polylactic Acid-Polyglycolic Acid Copolymer
Rabbits

Substances

Biocompatible Materials
Carbon Fiber
Polylactic Acid-Polyglycolic Acid Copolymer
Polyglycolic Acid
Lactic Acid
Carbon
Durapatite