In this paper, an iterative process is used in order to estimate the values of absorption coefficients of those materials of which little is known in the literature, so that an acoustic simulation can be carried out in Mudejar-Gothic churches. The estimation of the scattering coefficients, which is even less developed, is based on the size of the irregularities. This methodology implemented is applied to six Mudejar-Gothic churches of Seville (southern Spain). The simulated monophonic acoustic parameters, both in the frequency domain and as a function of source-receiver distance (spatial distribution), are analyzed and compared with the in situ measures. Good agreement has been found between these sets of values, whereby each parameter is discussed in terms of the just noticeable difference. This procedure for existing buildings, especially for those which are rich in heritage, enables a reliable evaluation of the effect on the maintenance, restoration, and conditioning for new uses, as well as the recreation of the acoustic environment of ancient times. Along these lines, the acoustic influence of the timber roof and the presence of the public in these churches have also been studied.