High-level resistance of Nosema ceranae, a parasite of the honeybee, to temperature and desiccation

Appl Environ Microbiol. 2009 Nov;75(21):6886-9. doi: 10.1128/AEM.01025-09. Epub 2009 Sep 4.

Abstract

Resistance of Nosema ceranae to different exposure conditions has been evaluated by using Sytox green and DAPI (4',6-diamidino-2-phenylindole) to test spore viability. High thermotolerance at 60 and 35 degrees C and resistance to desiccation were observed. However, a significant decrease in viability after freezing and a rapid degeneration of spores maintained at 4 degrees C were also detected.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bees / microbiology
  • Desiccation*
  • Freezing
  • Hot Temperature*
  • Indoles / metabolism
  • Microbial Viability*
  • Nosema / isolation & purification
  • Nosema / physiology*
  • Nosema / radiation effects*
  • Organic Chemicals / metabolism
  • Spores, Fungal / physiology
  • Spores, Fungal / radiation effects
  • Staining and Labeling / methods
  • Stress, Physiological*

Substances

  • Indoles
  • Organic Chemicals
  • SYTOX Green
  • DAPI