Post-embryonic plant growth and development are sustained by meristems, a source of undifferentiated cells that give rise to the adult plant structures. Two hormones, cytokinin and auxin, are known to act antagonistically in controlling meristem activities. Here, we review recent significant progress in elucidating the molecular mechanisms through which these hormones interact to control specific aspects of plant development. For example, in the root meristem of Arabidopsis thaliana, cytokinin promotes cell differentiation by repressing both auxin signalling and transport, whereas auxin sustains root meristem activity by promoting cell division. The coordinated action of these two hormones is essential for maintaining root meristem size and for ensuring root growth.