Preparation of a new chromogenic substrate to assay for beta-galactanases that hydrolyse type II arabino-3,6-galactans

Carbohydr Res. 2009 Oct 12;344(15):1941-6. doi: 10.1016/j.carres.2009.07.014. Epub 2009 Aug 12.

Abstract

A chromogenic assay using RB5-dGA, Reactive Black 5 (RB5) dye covalently coupled to de-arabinosylated gum arabic (dGA), was developed for rapid screening of beta-galactanases. dGA was prepared by partial acid hydrolysis (0.25M trifluoroacetic acid for 2h at 90-95 degrees C) of gum Arabic (GA) from Acacia senegal. The dGA exhibited a median molecular mass of approximately 10kDa, corresponding to a degree of polymerisation (DP) approximately 60. It was devoid of Ara residues, and contained mostly Galp (68mol%) together with GlcpA (30mol%). The Galp residues were (1,6)- (34mol%), (1,3)- (3mol%) and (1,3,6)- (26mol%) linked, and the GlcAp residues were primarily terminal (28mol%) together with a small amount of (1,4)-linked (2mol%), as expected for a type II (3,6)-galactan. The new chromogenic assay is simple, cost effective, relatively sensitive, and is specific for either beta-(1-->3)- and/or beta-(1-->6)-d-galactanases. It will enable routine large-scale screening of beta-galactanases from crude enzyme preparations and microorganism cultures, and is suitable for profiling activity during purification processes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromogenic Compounds / chemistry*
  • Chromogenic Compounds / metabolism*
  • Galactans / chemistry
  • Galactans / metabolism*
  • Glycoside Hydrolases / metabolism*
  • Hydrolysis
  • Molecular Structure

Substances

  • Chromogenic Compounds
  • Galactans
  • Glycoside Hydrolases
  • beta-galactanase