Severe pandemic (H1N1) 2009 influenza with false negative direct fluorescent antibody assay: case series

Luciano Kapelusznik; Rupa Patel; Jennifer Jao; Gopi Patel; Simon Daefler; Vincent Labombardi; David Calfee

doi:10.1016/j.jcv.2009.07.023

Severe pandemic (H1N1) 2009 influenza with false negative direct fluorescent antibody assay: case series

J Clin Virol. 2009 Nov;46(3):279-81. doi: 10.1016/j.jcv.2009.07.023. Epub 2009 Aug 29.

Authors

Luciano Kapelusznik¹, Rupa Patel, Jennifer Jao, Gopi Patel, Simon Daefler, Vincent Labombardi, David Calfee

Affiliation

¹ Division of Infectious Diseases, Mount Sinai School of Medicine, New York, NY 10029, United States. luciano.kapelusznik@mssm.edu

PMID: 19716765
DOI: 10.1016/j.jcv.2009.07.023

Abstract

Between May and June of 2009 we observed 4 patients that presented with severe influenza-like symptoms and respiratory failure. All cases tested negative for influenza A and B by direct fluorescent antibody assay. Further investigation revealed all cases to be positive for pandemic (H1N1) 2009 influenza virus by real-time RT-PCR. This article includes a description of these cases and the characteristics associated with them.

Publication types

Case Reports

MeSH terms

Adult
Aged
False Negative Reactions
Female
Fluorescent Antibody Technique, Direct / methods*
Humans
Influenza A Virus, H1N1 Subtype / genetics
Influenza A Virus, H1N1 Subtype / immunology
Influenza A Virus, H1N1 Subtype / isolation & purification*
Influenza, Human / diagnosis*
Influenza, Human / immunology
Influenza, Human / pathology
Influenza, Human / virology
Male
Middle Aged
Reverse Transcriptase Polymerase Chain Reaction / methods*