Objectives: The aim of this study is to investigate the effect of cristaloid cardioplegic fluid (CCF) and its contents on the susceptibility of plasma to copper-catalyzed lipid peroxidation test.
Design and methods: The plasma pool was divided into eight groups. Equal volumes of CCF or one of its contents were added to each group of the plasma pool. The accumulation of conjugated diene (CD) by copper-induced oxidation was monitored for a period of 5 h. Thiobarbituric acid-reactive substances (TBARS) formed during the incubation of plasma with copper was also measured.
Results: It was found that, the production of CD and TBARS were inhibited and the lag time had increased, when the plasma was mixed with CCF or its contents.
Conclusions: As a result, we conclude that that the susceptibility of plasma to copper-induced lipid peroxidation is interfered by CCF. The chloride ions, which major content of CCF, may play an important role on this effect.