Abstract
Tuberculous pleurisy, one of the most common manifestations of extrapulmonary tuberculosis, is characterized by a T-cell-mediated hypersensitivity reaction along with a Th1 immune profile. In this study, we investigated functional cross-talk among T and NK cells in human tuberculous pleurisy. We found that endogenously activated pleural fluid-derived NK cells express high ICAM-1 levels and induce T-cell activation ex vivo through ICAM-1. Besides, upon in vitro stimulation with monokines and PAMP, resting peripheral blood NK cells increased ICAM-1 expression leading to cellular activation and Th1 polarization of autologous T cells. Furthermore, these effects were abolished by anti-ICAM-1 Ab. Hence, NK cells may contribute to the adaptive immune response by a direct cell-contact-dependent mechanism in the context of Mycobacterium tuberculosis infection.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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CD11a Antigen / immunology
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CD56 Antigen / immunology
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Cell Communication / immunology
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Cysteine / analogs & derivatives
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Cysteine / pharmacology
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Humans
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Intercellular Adhesion Molecule-1 / drug effects
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Intercellular Adhesion Molecule-1 / immunology
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Intercellular Adhesion Molecule-1 / metabolism*
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Interleukin-12 / pharmacology
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Interleukin-15 / pharmacology
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Interleukin-18 / pharmacology
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Killer Cells, Natural / immunology*
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Lipopolysaccharides / pharmacology
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Lipoproteins / pharmacology
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Middle Aged
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Mycobacterium tuberculosis*
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Phosphotransferases (Phosphate Group Acceptor)
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T-Lymphocytes / immunology*
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Tuberculosis, Pleural / immunology*
Substances
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CD11a Antigen
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CD56 Antigen
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Interleukin-15
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Interleukin-18
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Lipopolysaccharides
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Lipoproteins
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Intercellular Adhesion Molecule-1
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Interleukin-12
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2,3-bis(palmitoyloxy)-2-propyl-1-palmitoylcysteine
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Phosphotransferases (Phosphate Group Acceptor)
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polyphosphate AMP phosphotransferase
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Cysteine