FM reconstruction of non-uniformly sampled protein NMR data at higher dimensions and optimization by distillation

J Biomol NMR. 2009 Nov;45(3):283-94. doi: 10.1007/s10858-009-9368-1. Epub 2009 Aug 25.

Abstract

Non-uniform sampling (NUS) enables recording of multidimensional NMR data at resolutions matching the resolving power of modern instruments without using excessive measuring time. However, in order to obtain satisfying results, efficient reconstruction methods are needed. Here we describe an optimized version of the Forward Maximum entropy (FM) reconstruction method, which can reconstruct up to three indirect dimensions. For complex datasets, such as NOESY spectra, the performance of the procedure is enhanced by a distillation procedure that reduces artifacts stemming from intense peaks.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Nuclear Magnetic Resonance, Biomolecular / methods*
  • Proteins / chemistry*
  • Signal Processing, Computer-Assisted

Substances

  • Proteins