The present study was carried out to determine the accumulation and variation of trace elements in roots and leaves of Asparagus racemosus collected from four different altitudes in Uttarakhand, India, by atomic absorption spectroscopy. The metals investigated were Zn, Cu, Mn, Fe, Co, Na, K, Ca, and Li. The concentration level of Fe was found to be highest at an altitude of 2,250 m, whereas the level of Cu was lowest. The maximum concentrations of Zn, Cu, Mn, Fe, Co, Na, K, Ca, and Li were found to be 165.0 +/- 3.2, 34.0 +/- 0.5, 84.0 +/- 0.7, 2,040.0 +/- 0.3, 122.0 +/- 1.5, 745.0 +/- 0.3, 13,260.0 +/- 3.5, 6,153.0 +/- 1.6, and 58.0 +/- 3.8 mg/kg, respectively.