The expanded-spectrum beta-lactamase (ESBL) gene bla(VEB-1), identified worldwide in Enterobacteriaceae and Pseudomonas aeruginosa, is associated with either class 1 integrons or repeated elements. We report here the first association of bla(VEB-1a) with the insertion sequence ISCR2 in six Acinetobacter species isolates recovered from Argentina. That genetic structure was likely at the origin of the mobilization of this ESBL gene.