Abstract
The glycosylation of alpha- and delta-tocopherols using Klebsiella pneumoniae and cyclodextrin glucanotransferase (CGTase) was investigated. K. pneumoniae converted alpha- and delta-tocopherols into the corresponding beta-glucosides in 10 and 8% yield, respectively. CGTase glycosylated alpha-tocopheryl beta-glucoside to alpha-tocopheryl beta-maltoside (51%) and alpha-tocopheryl beta-maltotrioside (35%). On the other hand, delta-tocopheryl beta-glucoside was converted into the corresponding beta-maltoside (45%) and beta-maltotrioside (29%) by CGTase. The beta-glucoside of alpha-tocopherol, and beta-glucoside and beta-maltoside of delta-tocopherol showed inhibitory effects on IgE antibody production and on histamine release from rat peritoneal mast cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Anti-Allergic Agents / chemistry*
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Anti-Allergic Agents / metabolism
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Anti-Allergic Agents / pharmacology
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Cell Line
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Glucosyltransferases / metabolism*
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Glycosylation
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Histamine Release / drug effects
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Klebsiella pneumoniae / metabolism*
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Mast Cells / drug effects
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Mast Cells / metabolism
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Oligosaccharides / chemistry*
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Oligosaccharides / metabolism
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Oligosaccharides / pharmacology
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Peritoneal Cavity / cytology
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Rats
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Tocopherols / chemistry
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Tocopherols / metabolism*
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alpha-Tocopherol / chemistry
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alpha-Tocopherol / metabolism*
Substances
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Anti-Allergic Agents
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Oligosaccharides
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maltooligosaccharides
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Glucosyltransferases
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cyclomaltodextrin glucanotransferase
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alpha-Tocopherol
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delta-tocopherol
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Tocopherols