Abstract
Commissural axons cross the ventral midline of the neural tube in a Slit-dependent manner. The underlying molecular mechanisms remain unclear. We found that the deubiquitinating enzyme USP33 interacts with the Robo1 receptor. USP33 was essential for midline crossing by commissural axons and for their response to Slit. Our results reveal a previously unknown role for USP33 in vertebrate commissural axon guidance and in Slit signaling.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Axons / physiology*
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Brain / embryology*
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Brain / metabolism
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Cell Line
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Cell Membrane / physiology
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Cells, Cultured
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Chick Embryo
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Growth Cones / physiology
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Humans
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Intercellular Signaling Peptides and Proteins / metabolism
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Mice
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Mice, Inbred ICR
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Mutation
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Nerve Tissue Proteins / genetics
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Nerve Tissue Proteins / metabolism
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Neurons / cytology
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Neurons / physiology
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Receptors, Immunologic / genetics
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Receptors, Immunologic / metabolism
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Roundabout Proteins
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Signal Transduction
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Spinal Cord / physiology
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Ubiquitin Thiolesterase / genetics
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Ubiquitin Thiolesterase / metabolism*
Substances
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Intercellular Signaling Peptides and Proteins
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Nerve Tissue Proteins
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Receptors, Immunologic
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USP33 protein, human
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Ubiquitin Thiolesterase
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Slit homolog 2 protein