Abstract
To examine whether the heat-labile enterotoxin gene in porcine enterotoxigenic Escherichia coli (ETEC) strains is as divergent as in human ETEC strains, we sequenced the heat-labile and heat-stable toxin genes from 52 and 33 porcine ETEC strains, respectively. We found that the STa gene is identical, that the LT gene has only two mutations in 4 (of 52) strains, and that both mutations cause a reduction in GM1 binding and toxicity.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Bacterial Toxins / genetics*
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DNA, Bacterial / genetics
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Enterotoxigenic Escherichia coli / genetics*
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Enterotoxigenic Escherichia coli / isolation & purification
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Enterotoxins / genetics*
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Escherichia coli Infections / microbiology
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Escherichia coli Infections / veterinary*
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Escherichia coli Proteins / genetics*
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Mutation, Missense
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Polymorphism, Genetic*
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Protein Binding
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Sequence Analysis, DNA
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Swine
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Swine Diseases / microbiology*
Substances
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Bacterial Toxins
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DNA, Bacterial
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Enterotoxins
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Escherichia coli Proteins
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heat stable toxin (E coli)
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heat-labile enterotoxin, E coli