Abstract
Conophylline, a vinca alkaloid extracted from the tropical plant Ervatamia microphylla, has been shown to induce the differentiation of insulin-producing beta-cells in cultured cells and in animals. However, its mechanism of action and the molecular target have remained unclear. Therefore, we prepared a fishing probe with conophylline to identify the target protein by using latex nano-beads, which are newly innovated tools for affinity-purification. With these conophylline-linked nano-beads, we found that conophylline directly interacted with ARL6IP. ARL6IP may thus be involved in the mechanism of cellular differentiation of beta-cells, and this probe should be useful to find other target proteins.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing / chemistry*
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Adaptor Proteins, Signal Transducing / metabolism
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Animals
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Cell Line, Tumor
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Hypoglycemic Agents / chemistry*
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Hypoglycemic Agents / metabolism
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Hypoglycemic Agents / pharmacology
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Insulin-Secreting Cells / cytology
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Membrane Proteins / chemistry*
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Membrane Proteins / metabolism
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Nanoparticles / chemistry*
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Plant Leaves / chemistry
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Plants, Medicinal / chemistry*
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Rats
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Recombinant Proteins / antagonists & inhibitors
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Recombinant Proteins / metabolism
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Vinca Alkaloids / chemistry*
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Vinca Alkaloids / metabolism
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Vinca Alkaloids / pharmacology
Substances
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Adaptor Proteins, Signal Transducing
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Arl6ip1 protein, rat
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Hypoglycemic Agents
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Membrane Proteins
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Recombinant Proteins
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Vinca Alkaloids
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conophylline