Induction of milk IgA antibodies by porcine respiratory coronavirus infection

P Callebaut; E Cox; M Pensaert; K Van Deun

doi:10.1007/978-1-4684-5823-7_58

Induction of milk IgA antibodies by porcine respiratory coronavirus infection

Adv Exp Med Biol. 1990:276:421-8. doi: 10.1007/978-1-4684-5823-7_58.

Authors

P Callebaut¹, E Cox, M Pensaert, K Van Deun

Affiliation

¹ Laboratory of Virology, Faculty of Veterinary Medicine, State University of Gent, Belgium.

PMID: 1966433
DOI: 10.1007/978-1-4684-5823-7_58

Abstract

An ELISA was developed to examine the prevalence of TGEV-specific immunoglobulin (Ig)A in the milk of sows, infected in the field with PRCV or with TGEV. It was shown that previous PRCV-infections can induce the secretion of IgA antibodies in the milk. However, only 9 out of 28 PRCV-infected sows had IgA in their milk whereas 11 TGEV-infected sows all secreted IgA. On farms where a reinfection with PRCV occurred, the number of IgA-secreting sows increased from 2 to 11 on a total of 13 sows. This showed that the presence of IgA antibodies in the milk may depend upon the occurrence of reinfection with PRCV. As demonstrated by density gradient analysis, the milk IgA induced by PRCV was 11S secretory IgA and had the capacity to neutralize TGEV.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies, Viral / analysis*
Antibodies, Viral / biosynthesis
Antibody Formation
Coronaviridae / immunology*
Coronaviridae Infections / immunology*
Enzyme-Linked Immunosorbent Assay
Female
Gastroenteritis, Transmissible, of Swine / immunology*
Immunoglobulin A / analysis*
Immunoglobulin A / biosynthesis
Milk / immunology*
Neutralization Tests
Swine
Transmissible gastroenteritis virus / immunology*

Substances

Antibodies, Viral
Immunoglobulin A