In vitro covalent binding proteins of zerumbone, a chemopreventive food factor

Biosci Biotechnol Biochem. 2009 Aug;73(8):1905-7. doi: 10.1271/bbb.90265. Epub 2009 Aug 7.

Abstract

Zerumbone (ZER), a sesquiterpene in Zingiber zerumbet Smith, has been implicated as a promising chemopreventive agent. Here we found that ZER suppressed expression of pro-inflammatory genes (COX-2 and iNOS) and induced detoxification genes (GSTP1 and NQO1) in RAW264.7 macrophages. Using the ZER-bound Sepharose gel, it appeared ZER was covalently bound to proteins, Keap1 and HuR, that play key roles in these molecular mechanisms.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / chemistry
  • Anti-Inflammatory Agents / metabolism
  • Anti-Inflammatory Agents / pharmacology
  • Cell Line
  • Chemoprevention
  • Food*
  • Gene Expression Regulation / drug effects
  • Humans
  • Macrophages / drug effects
  • Macrophages / metabolism
  • Mice
  • Protein Binding
  • Proteins / analysis*
  • Proteins / chemistry
  • Proteins / metabolism*
  • Rats
  • Sesquiterpenes / chemistry
  • Sesquiterpenes / metabolism*
  • Sesquiterpenes / pharmacology*
  • Sulfhydryl Compounds / chemistry
  • Zingiberaceae / chemistry*

Substances

  • Anti-Inflammatory Agents
  • Proteins
  • Sesquiterpenes
  • Sulfhydryl Compounds
  • zerumbone