Isoptericola jiangsuensis sp. nov., a chitin-degrading bacterium

Int J Syst Evol Microbiol. 2010 Apr;60(Pt 4):904-908. doi: 10.1099/ijs.0.012864-0. Epub 2009 Aug 6.

Abstract

A Gram-positive-staining, non-motile, rod- or coccoid-shaped bacterium, strain CLG(T), was isolated from beach sand from Lianyungang, Jiangsu Province, PR China. The organism grew optimally at 30 degrees C and pH 7.0. Strain CLG(T) contained L-Lys-D-Asp as the peptidoglycan type and galactose, rhamnose and xylose as the whole-cell sugars. It contained anteiso-C(15 : 0) and iso-C(15 : 0) as the major fatty acids. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phospholipid, phosphatidylinositol, three phosphoglycolipids and one unidentified lipid. The DNA G+C content was 70.3 mol%. The 16S rRNA gene sequence similarity of strain CLG(T) with Isoptericola dokdonensis DS-3(T) was 99.4 % but the DNA-DNA relatedness between strain CLG( T) and I. dokdonensis DS-3(T) was 58.7 %. There were also many phenotypic properties that distinguished strain CLG( T) from recognized species of the genus Isoptericola. The results showed that strain CLG(T) represents a novel species of the genus Isoptericola, for which the name Isoptericola jiangsuensis sp. nov. is proposed. The type strain is CLG(T) (=DSM 21863( T)=CCTCC AB208287(T)).

MeSH terms

  • Actinomycetales / classification*
  • Actinomycetales / genetics
  • Actinomycetales / isolation & purification
  • Actinomycetales / metabolism
  • Bacterial Typing Techniques
  • Base Composition
  • Bathing Beaches*
  • China
  • Chitin / metabolism*
  • DNA, Ribosomal / analysis
  • DNA, Ribosomal / genetics
  • Fatty Acids / analysis
  • Genes, rRNA
  • Genotype
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Phenotype
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Silicon Dioxide*
  • Soil Microbiology*
  • Species Specificity

Substances

  • DNA, Ribosomal
  • Fatty Acids
  • RNA, Ribosomal, 16S
  • Chitin
  • Silicon Dioxide

Associated data

  • GENBANK/EU852101