Protective effect on enamel demineralization of a CPP-ACP paste: an AFM in vitro study

J Dent. 2009 Dec;37(12):949-54. doi: 10.1016/j.jdent.2009.07.011. Epub 2009 Aug 4.

Abstract

Objectives: The aim of the present in vitro study was the evaluation of a CPP-ACP paste (Tooth Mousse) on preventing dental erosion produced by a soft drink (Coca Cola), using Atomic Force Microscopy (AFM).

Methods: Thirty extracted human central incisors free of caries were selected and divided in a treatment and a control half. The treatment halves were divided in three groups-group 1: demineralization with soft drink (4 intervals of 2 min); group 2: demineralization with soft drink (4 intervals of 2 min) plus Tooth Mousse; group 3: intact enamel plus Tooth Mousse. In groups 2 and 3 Tooth Mousse was applied for 3 min at 0, 8, 24 and 36 h. The surface of each specimen was imaged by AFM and R(rms) values were registered.

Results: Among treatment specimens of groups 1 and 2, a statistically significant difference (P<0.01) in R(rms) values was registered: treatment of the specimens with CPP-ACP paste had a protective effect on enamel demineralization. In group 3 no statistically significant difference was registered between exposed and not exposed halves of the specimens.

Conclusions: The use of a CPP-ACP paste had a protective effect on enamel demineralization in an in vitro model.

Publication types

  • Comparative Study
  • Randomized Controlled Trial

MeSH terms

  • Carbonated Beverages / adverse effects
  • Caseins / therapeutic use*
  • Dental Enamel / drug effects*
  • Dental Enamel / ultrastructure
  • Humans
  • Hydrogen-Ion Concentration
  • Image Processing, Computer-Assisted
  • Materials Testing
  • Microscopy, Atomic Force
  • Protective Agents / therapeutic use*
  • Temperature
  • Time Factors
  • Tooth Demineralization / prevention & control*
  • Tooth Erosion / prevention & control*
  • Tooth Remineralization / methods

Substances

  • Caseins
  • Protective Agents
  • casein phosphopeptide-amorphous calcium phosphate nanocomplex