Antimicrobial susceptibility, beta-lactamase and enterotoxin production in Bacillus cereus isolates from clinical and food samples

Folia Microbiol (Praha). 2009;54(3):233-8. doi: 10.1007/s12223-009-0037-2. Epub 2009 Aug 2.

Abstract

The antimicrobial susceptibility of 30 clinical and 30 food Bacillus cereus isolates was determined. All isolates were susceptible to streptomycin, ciprofloxacin and gentamicin, 90 % of them to clindamycin and vancomycin, and 67 % to erythromycin. All isolates were resistant to amoxicillin with clavulanic acid, ampicillin, cefotaxime, ciprofloxacin, cloxacillin, cefotaxime with clavulanic acid and penicillin. The MIC values (determined by E-tests) were 48-256 mg/L for ampicillin, 0.19-1.5 mg/L for gentamicin, 0.125-1.0 mg/L for clindamycin, 0.047-4.0 mg/L for erythromycin and 1.5-16 mg/L for vancomycin. The MICs 4.6-18.75 g/L were observed for penicillin using the microdilution method. The presence of metallo-beta-lactamases was detected by E-test for 100 % of strains. Nonhemolytic diarrheal enterotoxin (NHE) was produced by 98.3 % of strains, while 31.7 % of them produced hemolytic diarrheal enterotoxin (HBL). Clinical isolates produced 10 % more HBL than food isolates. The psychrotrophic strains isolated from food samples produced NHE at 6.5 degrees C in 73 % of cases.

Publication types

  • Comparative Study

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • Bacillus cereus / genetics
  • Bacillus cereus / isolation & purification
  • Bacillus cereus / metabolism*
  • Drug Resistance, Multiple, Bacterial*
  • Enterotoxins / metabolism*
  • Food Contamination*
  • Gram-Positive Bacterial Infections / microbiology*
  • Humans
  • Microbial Sensitivity Tests
  • Temperature
  • beta-Lactamases / metabolism*

Substances

  • Anti-Bacterial Agents
  • Enterotoxins
  • beta-Lactamases