Evaluation of two real-time PCR chemistries for the detection of Chlamydophila pneumoniae in clinical specimens

Stephanie L Mitchell; Sona Budhiraja; Kathleen A Thurman; W Lanier Thacker; Jonas M Winchell

doi:10.1016/j.mcp.2009.07.005

Evaluation of two real-time PCR chemistries for the detection of Chlamydophila pneumoniae in clinical specimens

Mol Cell Probes. 2009 Dec;23(6):309-11. doi: 10.1016/j.mcp.2009.07.005. Epub 2009 Jul 30.

Authors

Stephanie L Mitchell¹, Sona Budhiraja, Kathleen A Thurman, W Lanier Thacker, Jonas M Winchell

Affiliation

¹ Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA.

PMID: 19647071
DOI: 10.1016/j.mcp.2009.07.005

Abstract

Chlamydophila pneumoniae is an atypical bacterial respiratory pathogen that is responsible for approximately 3-10% of community-acquired pneumonia cases. We report the evaluation of two distinct real-time PCR assays for rapid and specific detection of C. pneumoniae. We tested 401 clinical specimens, finding 5.7% positive, and confirmed a localized outbreak.

Publication types

Evaluation Study

MeSH terms

Chlamydophila Infections / diagnosis
Chlamydophila Infections / microbiology
Chlamydophila pneumoniae / genetics*
DNA Primers
DNA, Bacterial / genetics*
Humans
Oligonucleotide Probes
Pneumonia, Bacterial / diagnosis
Pneumonia, Bacterial / microbiology
Polymerase Chain Reaction / methods*
Reproducibility of Results
Sensitivity and Specificity

Substances

DNA Primers
DNA, Bacterial
Oligonucleotide Probes