Steroidal glycosides from Agave utahensis and their cytotoxic activity

Akihito Yokosuka; Maki Jitsuno; Satoru Yui; Masatoshi Yamazaki; Yoshihiro Mimaki

doi:10.1021/np900168d

Steroidal glycosides from Agave utahensis and their cytotoxic activity

J Nat Prod. 2009 Aug;72(8):1399-404. doi: 10.1021/np900168d.

Authors

Akihito Yokosuka¹, Maki Jitsuno, Satoru Yui, Masatoshi Yamazaki, Yoshihiro Mimaki

Affiliation

¹ Laboratory of Medicinal Pharmacognosy, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Horinouchi 1432-1, Hachiouji, Tokyo 192-0392, Japan. yokosuka@toyaku.ac.jp

PMID: 19645463
DOI: 10.1021/np900168d

Abstract

Eight new spirostanol saponins (1-8) and three new furostanol saponins (9-11) were isolated from the whole plants of Agave utahensis. The structures of 1-11 were determined by analysis of extensive spectroscopic data. The saponins were evaluated for their cytotoxic activity against HL-60 human promyelocytic leukemia cells. Compound 1 showed cytotoxicity against HL-60 cells with an IC(50) value of 4.9 microg/mL, induced apoptosis in HL-60 cells, and markedly activated caspase-3.

MeSH terms

Agave / chemistry*
Antineoplastic Agents, Phytogenic / chemistry
Antineoplastic Agents, Phytogenic / isolation & purification*
Antineoplastic Agents, Phytogenic / pharmacology*
Caspase 3 / drug effects
Drug Screening Assays, Antitumor
HL-60 Cells
Humans
Molecular Structure
Nuclear Magnetic Resonance, Biomolecular
Plants, Medicinal / chemistry*
Saponins / chemistry
Saponins / isolation & purification*
Saponins / pharmacology*
Spirostans / chemistry
Spirostans / isolation & purification*
Spirostans / pharmacology*
Stereoisomerism

Substances

Antineoplastic Agents, Phytogenic
Saponins
Spirostans
Caspase 3