Recently, the measurement of biochemical bone turnover markers by automated immunoassay analyzer system was performed for the routine clinical laboratory tests in patients with osteoporosis and other metabolic bone diseases. Measurement of serum and/or urine bone turnover markers has been reported to be easy assay, rapid measurement and highly precision for routine tests using the automated immunoassay analyzer system. These measurements may be enabled as cheap routine tests in the future for all medical institution if automated measurement of the bone turnover marker spreading for medical examination and treatment. In conclusion, it indicates that the measurement of automated immunoassay analyzer system may be applicable for routine measurement and provide a useful tool to bone turnover markers.