Sequence-specific 1H, 15N and 13C resonance assignments of the 23.7-kDa homodimeric toxin CcdB from Vibrio fischeri

Michal Respondek; Lieven Buts; Natalie De Jonge; Sarah Haesaerts; Remy Loris; Laurence Van Melderen; Lode Wyns; Klaus Zangger

doi:10.1007/s12104-009-9161-9

Sequence-specific 1H, 15N and 13C resonance assignments of the 23.7-kDa homodimeric toxin CcdB from Vibrio fischeri

Biomol NMR Assign. 2009 Jun;3(1):145-7. doi: 10.1007/s12104-009-9161-9. Epub 2009 Apr 3.

Authors

Michal Respondek¹, Lieven Buts, Natalie De Jonge, Sarah Haesaerts, Remy Loris, Laurence Van Melderen, Lode Wyns, Klaus Zangger

Affiliation

¹ Institute of Chemistry/Organic and Bioorganic Chemistry, University of Graz, Heinrichstrasse 28, 8010, Graz, Austria.

PMID: 19636967
DOI: 10.1007/s12104-009-9161-9

Abstract

CcdB is the toxic component of a bacterial toxin-antitoxin system. It inhibits DNA gyrase (a type II topoisomerase), and its toxicity can be neutralized by binding of its antitoxin CcdA. Here we report the sequential backbone and sidechain (1)H, (15)N and (13)C resonance assignments of CcdB(Vfi) from the marine bacterium Vibrio fischeri. The BMRB accession number is 16135.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aliivibrio fischeri / chemistry*
Amino Acid Sequence
Bacterial Proteins / chemistry*
Bacterial Toxins / chemistry*
Carbon Isotopes / chemistry
Magnetic Resonance Spectroscopy / methods*
Molecular Sequence Data
Molecular Weight
Nitrogen Isotopes / chemistry
Protein Structure, Tertiary
Protein Subunits
Protons

Substances

Bacterial Proteins
Bacterial Toxins
Carbon Isotopes
CcdB protein, Plasmid F
Nitrogen Isotopes
Protein Subunits
Protons