Coordinated control of a designed trans-acting ligase ribozyme by a loop-receptor interaction

Shigeyoshi Matsumura; Rei Ohmori; Hirohide Saito; Yoshiya Ikawa; Tan Inoue

doi:10.1016/j.febslet.2009.07.036

Coordinated control of a designed trans-acting ligase ribozyme by a loop-receptor interaction

FEBS Lett. 2009 Sep 3;583(17):2819-26. doi: 10.1016/j.febslet.2009.07.036. Epub 2009 Jul 23.

Authors

Shigeyoshi Matsumura¹, Rei Ohmori, Hirohide Saito, Yoshiya Ikawa, Tan Inoue

Affiliation

¹ Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto, Japan.

PMID: 19631647
DOI: 10.1016/j.febslet.2009.07.036

Abstract

We previously developed a synthetic cis-acting RNA ligase ribozyme with 3'-5' joining activity termed "DSL" (designed and selected ligase). DSL was easily transformed into a trans-acting form because of its highly modular architecture. In this study, we investigated the modular properties and turnover capabilities of a trans-acting DSL, tDSL-1/GUAA. tDSL-1/GUAA exhibited remarkably high activity compared with the parental cis-acting DSL, and it attained a high turnover number. Taken together, the results indicate that a loop-receptor interaction plays a significant role in determining the activity of the trans-acting ribozyme and in its ability to perform multiple turnovers of the reaction.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Pairing
Base Sequence
Molecular Sequence Data
Nucleic Acid Conformation*
RNA Ligase (ATP) / chemistry*
RNA Ligase (ATP) / genetics
RNA Ligase (ATP) / metabolism*
RNA, Catalytic / chemistry*
RNA, Catalytic / genetics
RNA, Catalytic / metabolism*
Substrate Specificity

Substances

RNA, Catalytic
RNA Ligase (ATP)