Insights on nuclease mechanism: the role of proximal ammonium group on phosphate esters cleavage

Renato Bonomi; Giacomo Saielli; Umberto Tonellato; Paolo Scrimin; Fabrizio Mancin

doi:10.1021/ja9033236

Insights on nuclease mechanism: the role of proximal ammonium group on phosphate esters cleavage

J Am Chem Soc. 2009 Aug 19;131(32):11278-9. doi: 10.1021/ja9033236.

Authors

Renato Bonomi¹, Giacomo Saielli, Umberto Tonellato, Paolo Scrimin, Fabrizio Mancin

Affiliation

¹ Dipartimento di Scienze Chimiche and CNR-ITM, Universita di Padova, via Marzolo 1, I-35131 Padova, Italy.

PMID: 19627082
DOI: 10.1021/ja9033236

Abstract

The role of remotely located ammonium groups in the active site of many phosphate-cleaving enzymes is investigated by using model systems thus providing mechanistic hints to a still unsolved problem.

MeSH terms

Catalytic Domain
Endonucleases / chemistry
Endonucleases / metabolism*
Models, Molecular
Organophosphates / metabolism*
Quaternary Ammonium Compounds / chemistry
Quaternary Ammonium Compounds / metabolism*

Substances

Organophosphates
Quaternary Ammonium Compounds
Endonucleases