Abstract
We compared a DNA-based assay with a total nucleic acid-based assay for early detection of infant human immunodeficiency virus (HIV) infection. The codetection of DNA and RNA did not result in an overall higher sensitivity compared to that of DNA alone. Discordant results were associated with low levels of HIV DNA, indicating that the sample amount may be critical.
Publication types
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Comparative Study
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Evaluation Study
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Research Support, N.I.H., Extramural
MeSH terms
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Blood / virology*
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DNA, Viral / blood*
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Desiccation*
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Early Diagnosis
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HIV / isolation & purification*
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HIV Infections / diagnosis*
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HIV Infections / virology
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Humans
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Infant
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Infant, Newborn
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RNA, Viral / blood*
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Sensitivity and Specificity
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Specimen Handling / methods*