Abstract
We report here a new method for inhibition of RNA viruses induced by dsDNA. We demonstrated that both long dsDNA molecules and short interfering DNA with a sequence complementary to that of viral RNA inhibited tobacco mosaic virus expression and prevented virus spread. Also, the expression of the HIV-1 gp41 gene in HeLa cells was inhibited by complementary short interfering DNA. We showed that Dicer processed dsDNA, which suggests activation of the cellular machinery involved in silencing of RNA. For the silencing of viral RNA effected with dsDNA, we coined the term DNA interference technology.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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DEAD-box RNA Helicases / physiology
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DNA, Antisense / genetics
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DNA, Antisense / pharmacology*
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Gene Silencing / drug effects*
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Genes, Viral / drug effects*
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HIV Envelope Protein gp41 / antagonists & inhibitors
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HIV Envelope Protein gp41 / genetics
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HeLa Cells
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Humans
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Methods
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Nucleic Acid Hybridization / methods
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Oligodeoxyribonucleotides, Antisense / genetics
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Oligodeoxyribonucleotides, Antisense / pharmacology
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RNA Viruses / drug effects*
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RNA Viruses / genetics
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RNA, Viral / antagonists & inhibitors*
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Ribonuclease III / physiology
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Tobacco Mosaic Virus / drug effects
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Tobacco Mosaic Virus / genetics
Substances
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DNA, Antisense
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HIV Envelope Protein gp41
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Oligodeoxyribonucleotides, Antisense
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RNA, Viral
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DICER1 protein, human
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Ribonuclease III
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DEAD-box RNA Helicases