Abstract
Macrophages are central players in both lipid metabolism and innate immunity. Their determinant role in the pathogenesis of atherosclerosis is under the control of the ATP-binding cassette transporter (ABCA1), which by minimizing cellular lipid content, limits development of pro-inflammatory foam cells. Considering the differential contribution of monocyte subsets to the generation of vascular lesions we analyzed the immunophenotype of ABCA1-expressing cells in the myeloid lineage, by the combined use of flow cytometry and real-time quantitative RT-PCR. ABCA1 expression is limited to "non-inflammatory" Ly6C(lo) circulating monocytes and tissue-resident macrophages expressing markers of alternative activation. In ABCA1(-/-) peritoneal macrophages the transcriptional programs induced by LPS/IFN-gamma or IL-4 cytokines are altered and deviated phosphorylation patterns of STAT transcriptional regulators in response to stimuli are observed.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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ATP Binding Cassette Transporter 1
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ATP-Binding Cassette Transporters / genetics
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ATP-Binding Cassette Transporters / metabolism*
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Animals
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism
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Blotting, Western
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Cells, Cultured
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Cyclooxygenase 2 / metabolism
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Cytokines / pharmacology*
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Flow Cytometry
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Gene Expression Profiling
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HeLa Cells
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Humans
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Immunophenotyping
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Interferon-gamma / pharmacology
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Interleukin-4 / pharmacology
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Luminescent Proteins / genetics
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Luminescent Proteins / metabolism
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Macrophages / cytology
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Macrophages / drug effects*
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Macrophages / metabolism
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Macrophages, Peritoneal / cytology
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Macrophages, Peritoneal / drug effects
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Macrophages, Peritoneal / metabolism
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Mice
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Mice, Inbred BALB C
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Mice, Inbred C57BL
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Mice, Inbred DBA
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Mice, Knockout
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Phosphorylation / drug effects
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Reverse Transcriptase Polymerase Chain Reaction
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STAT1 Transcription Factor / metabolism
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Species Specificity
Substances
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ABCA1 protein, human
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ATP Binding Cassette Transporter 1
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ATP-Binding Cassette Transporters
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Bacterial Proteins
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Cytokines
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Luminescent Proteins
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STAT1 Transcription Factor
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yellow fluorescent protein, Bacteria
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Interleukin-4
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Interferon-gamma
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Ptgs2 protein, mouse
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Cyclooxygenase 2