The enantiomeric fractions present in soil samples may provide information useful in distinguishing recent inputs of DDT from past DDT pollution. In this study, a chromatographic procedure for the determination of the enantiomeric fractions of o,p'-DDT based on heart-cutting multidimensional gas chromatography was developed. The optimization carried out achieved low ratios of DDT degradation (<15%) in the chromatographic system. High selectivity and sensitivity in the detection of the target compounds, with a limit of detection as low as 2.1 pg microL(-1), was reached. In addition, high degrees of repeatability (RSD<2.0%) and reproducibility (RSD<3.2%) were obtained for the enantiomeric fractions measured in analytical standards and soil samples.