Detection of staphylococcal enterotoxin and toxic shock syndrome toxin-1 (TSST-1) by immunoblot combined with a semiautomated electrophoresis system

J A Orden; J Goyache; F J Hernández; A Doménech; G Suárez; E Gómez-Lucía

doi:10.1016/0022-1759(91)90086-u

Detection of staphylococcal enterotoxin and toxic shock syndrome toxin-1 (TSST-1) by immunoblot combined with a semiautomated electrophoresis system

J Immunol Methods. 1991 Nov 22;144(2):197-202. doi: 10.1016/0022-1759(91)90086-u.

Authors

J A Orden¹, J Goyache, F J Hernández, A Doménech, G Suárez, E Gómez-Lucía

Affiliation

¹ Dpto. Patología Animal I, Facultad de Veterinaria, Universidad Complutense de Madrid, Spain.

PMID: 1960416
DOI: 10.1016/0022-1759(91)90086-u

Abstract

An immunoblot technique combined with a semi-automated electrophoresis system has been developed for the detection of staphylococcal enterotoxins and toxic shock syndrome toxin-1 (TSST-1). The advantages of this method over other detection techniques include speed, sensitivity (10 ng/ml) and specificity. The use of semiautomated electrophoresis permits the routine detection of staphylococcal enterotoxins and TSST-1.

MeSH terms

Bacterial Toxins*
Electrophoresis, Polyacrylamide Gel
Enterotoxins / analysis*
Enzyme-Linked Immunosorbent Assay
Immunoblotting
Molecular Weight
Staphylococcus aureus*
Superantigens*

Substances

Bacterial Toxins
Enterotoxins
Superantigens
enterotoxin F, Staphylococcal