Cardenolide glycosides of Thevetia peruviana and triterpenoid saponins of Sapindus emarginatus as TRAIL resistance-overcoming compounds

J Nat Prod. 2009 Aug;72(8):1507-11. doi: 10.1021/np900202n.

Abstract

A screening study for TRAIL resistance-overcoming activity was carried out, and activity-guided fractionations of Thevetia peruviana and Sapindus emarginatus led to the isolation of four cardenolide glycosides (1-4) and four triterpenoid saponins (5-8), respectively. In particular, cardenolide glycosides (1 and 2) from T. peruviana were shown to have a significant reversal effect on TRAIL resistance in human gastric adenocarcinoma cells, and real-time PCR showed that thevefolin (2) enhanced mRNA expression of death receptor 4 (DR4) and DR5. In addition, 1H and 13C NMR characterizations are shown for thevefolin (2) for the first time.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Cardenolides / chemistry
  • Cardenolides / isolation & purification*
  • Cardenolides / pharmacology*
  • Humans
  • Molecular Structure
  • Nuclear Magnetic Resonance, Biomolecular
  • Plants, Medicinal / chemistry*
  • Receptors, TNF-Related Apoptosis-Inducing Ligand / genetics
  • Sapindus / chemistry*
  • Saponins / chemistry
  • Saponins / isolation & purification*
  • Saponins / pharmacology*
  • Stomach Neoplasms / metabolism
  • TNF-Related Apoptosis-Inducing Ligand / drug effects*
  • Thailand
  • Thevetia / chemistry*
  • Tumor Cells, Cultured

Substances

  • Cardenolides
  • Receptors, TNF-Related Apoptosis-Inducing Ligand
  • Saponins
  • TNF-Related Apoptosis-Inducing Ligand