Abstract
Active inclusion bodies of polyphosphate kinase 3 and cytidine 5'-monophosphate kinase were combined with whole cells that co-express sialic acid aldolase and CMP-sialic acid synthetase. The biocatalytic mixture was used for the synthesis of CMP-sialic acid, which was then converted to 3'-sialyllactose by whole cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Biocatalysis
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Cytidine Monophosphate N-Acetylneuraminic Acid / chemistry
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Cytidine Monophosphate N-Acetylneuraminic Acid / metabolism*
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Molecular Structure
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Nucleoside-Diphosphate Kinase / metabolism
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Oligosaccharides / chemistry
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Oligosaccharides / metabolism
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Phosphotransferases (Phosphate Group Acceptor) / metabolism*
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Rhodobacteraceae / enzymology*
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Substrate Specificity
Substances
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3'-sialyllactose
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Oligosaccharides
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Cytidine Monophosphate N-Acetylneuraminic Acid
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Phosphotransferases (Phosphate Group Acceptor)
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polyphosphate kinase
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Nucleoside-Diphosphate Kinase