Two different case studies concerning potential overload situations of anaerobic digesters were investigated and mathematically modelled by means of the Anaerobic Digestion Model No. 1 (ADM1). The first scenario included a digester failure at a municipal WWTP which occurred during revision works of the upstream digester within a two-step digestion system when the sludge was directly by-passed to the 2nd-step reactor. Secondly, the non-occurrence of a highly expected upset situation in a lab-scale digester fed with cattle manure was investigated. ADM1 was utilized to derive indicators which were used to investigate the relationship between digester stability and biomass population dynamics. Conventional design parameters such as the organic loading rate appeared unsuitable for process description under dynamic conditions. Indicators reflecting the biokinetic state (e.g. F(net)/M(net) or the VFA/alkalinity ratio) are more adequate for the assessment of the stability of reactors in transient situations.