Abstract
In this unit, we describe a set of improvements we have made to the standard Illumina Genome Analyzer protocols to make the sequencing process more reliable in a high-throughput environment, reduce amplification bias, narrow the distribution of insert sizes, and reliably obtain high yields of data.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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DNA / analysis
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DNA / genetics
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Electrophoresis, Agar Gel
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Gene Library
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Genome*
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Humans
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Nucleic Acid Denaturation
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Polymerase Chain Reaction / methods
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Sequence Analysis, DNA* / instrumentation
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Sequence Analysis, DNA* / methods
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Templates, Genetic