The main objective of this study was to evaluate the frequency distribution of mycobiota and the concentration of Ochratoxin A (OTA) in 50 samples from one company of commercial brand poultry feed produced in Venezuela. The concentration of OTA in the samples analyzed was determined using the competitive ELISA method. The most frequently isolated genera of moulds were Aspergillus (36%) and Penicillium (20%). Of these genera, the most frequently isolated species were Aspergillus flavus, Aspergillus terreus and Penicillium citrinum. Ochratoxigenic species such as Eurotium herbariorum, Aspergillus niger, Aspergillus ochraceus and Aspergillus glaucus, were also found with lower frequency. Rhodotorula mucilaginosa was the only yeast isolated. 94% of the samples presented contamination by OTA in a range between 2.558 and 31.978 microg kg(-1) feed and 42% of them presented OTA levels from 10 up to 20 microg kg(-1). The findings of this investigation show that 84% of the samples of concentrated feed for meat poultry surpass the maximum permitted limit for OTA of 5 microg kg(-1), established in the majority of countries in which regulations are placed.