Abstract
We developed a metal ion chelate-assisted ligation for SNP detection by microarray. An oligonucleotide probe was separated into two 9-10-mers bearing iminodiacetic residues at the gap point. Duplex formation with the DNA target was possible only if nickel ions were added, but a nucleotide substitution opposite the gap point prevented duplex formation. Here we demonstrate the application of this approach for SNP detection (A1298C) within the 5,10-methylenetetrahydrofolate reductase gene on a microarray.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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Chelating Agents / chemistry
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DNA / chemistry*
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DNA Probes / chemistry
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Humans
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Ions / chemistry*
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Metals / chemistry*
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Microchip Analytical Procedures / methods*
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Molecular Sequence Data
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Nickel / chemistry
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Oligonucleotide Array Sequence Analysis
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Oligonucleotides
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Polymorphism, Single Nucleotide*
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Solubility
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Tetrahydrofolates / chemistry
Substances
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Chelating Agents
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DNA Probes
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Ions
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Metals
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Oligonucleotides
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Tetrahydrofolates
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5,10-methylenetetrahydrofolic acid
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Nickel
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DNA