Metal ion CHElate-aSSisted LIGAtion (CHESS LIGA) for SNP detection on microarrays

Bioorg Med Chem Lett. 2009 Aug 1;19(15):4018-21. doi: 10.1016/j.bmcl.2009.06.033. Epub 2009 Jun 13.

Abstract

We developed a metal ion chelate-assisted ligation for SNP detection by microarray. An oligonucleotide probe was separated into two 9-10-mers bearing iminodiacetic residues at the gap point. Duplex formation with the DNA target was possible only if nickel ions were added, but a nucleotide substitution opposite the gap point prevented duplex formation. Here we demonstrate the application of this approach for SNP detection (A1298C) within the 5,10-methylenetetrahydrofolate reductase gene on a microarray.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Chelating Agents / chemistry
  • DNA / chemistry*
  • DNA Probes / chemistry
  • Humans
  • Ions / chemistry*
  • Metals / chemistry*
  • Microchip Analytical Procedures / methods*
  • Molecular Sequence Data
  • Nickel / chemistry
  • Oligonucleotide Array Sequence Analysis
  • Oligonucleotides
  • Polymorphism, Single Nucleotide*
  • Solubility
  • Tetrahydrofolates / chemistry

Substances

  • Chelating Agents
  • DNA Probes
  • Ions
  • Metals
  • Oligonucleotides
  • Tetrahydrofolates
  • 5,10-methylenetetrahydrofolic acid
  • Nickel
  • DNA