Objective: To study the effect on bioactive components of Xuesaitong injection in the process of ultra-filter by orthogonal test.
Methods: The change of the contents of active components was examined by HPLC method, using notoginsennoside R1 , ginsennoside Rg1, ginsennoside Rb1 and ginsennoside Rd as the mark components. The effect of the transmissivity of active components was studied with four different membrane parameters (such as pore diameter of membrane, operational pressure, the temperature and the concentration of the officinal liquid) by orthogonal test.
Results: In the process of ultra-filter the transmissivity of notoginsennoside R, and ginsennoside Rg, had the same characteristics. Both of the average transmissivities of these two components were in the range of 45% to 95%. The concentration of the officinal liquid showed significant influence. The transmissivities of ginsennoside Rb, and ginsennoside Rd in the process of ultra-filiter had the same characteristic. The average transmissivities of these two components were in the range of 2 8%-62% and 1.8%-45%, respectively. The pore diameter of membrane and the temperature showed significant influence. The concentration of the officinal liquid also showed significant influence on the transmissivity of ginsennoside Rd.
Conclusion: This study provides an external direction for the preparation of Xuesaitong injection.