Abstract
A method for the construction of West Nile virus immune donor antibody repertoires is described. B cells are harvested from a suitable donor and the antibody variable genes are amplified using polymerase chain reaction (PCR). The PCR fragments are cloned in a phage display vector to construct a repertoire that can be used in panning procedures to identify many unique monoclonal antibodies.
MeSH terms
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Antibodies, Viral / immunology*
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Antigens, Viral / immunology
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Bacteriophages / immunology*
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Blood Donors*
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Humans
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Immunoglobulin Heavy Chains / genetics
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Immunoglobulin Heavy Chains / immunology
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Immunoglobulin Light Chains / genetics
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Immunoglobulin Light Chains / immunology
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Immunoglobulin Variable Region / genetics
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Immunoglobulin Variable Region / immunology
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Peptide Library*
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Protein Engineering
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RNA, Viral / genetics
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RNA, Viral / metabolism
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West Nile Fever / blood*
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West Nile virus / immunology*
Substances
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Antibodies, Viral
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Antigens, Viral
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Immunoglobulin Heavy Chains
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Immunoglobulin Light Chains
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Immunoglobulin Variable Region
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Peptide Library
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RNA, Viral