Abstract
Mice were immunized either with unadjuvanted seasonal trivalent influenza vaccine (TIV) or TIV formulated with Vaxfectin, a cationic lipid-based adjuvant. Increasing doses of Vaxfectin resulted in increased hemagglutination-inhibition or anti-TIV ELISA antibody titers, with up to a 200-fold increase obtained with 900 microg of Vaxfectin. A >or=10-fold dose-sparing effect was demonstrated with Vaxfectin formulations. Vaxfectin preferentially increased IgG2 titers compared to IgG1 titers, resulting in a balanced IgG isotype distribution. Lower doses of Vaxfectin (30 microg) did not enhance antibody responses, but increased the number of IFN-gamma secreting T-cells by up to 18-fold. The data demonstrate that Vaxfectin enhances Th1 responses with protein-based seasonal influenza vaccine, and suggest that cellular or humoral immune responses may be preferentially induced by modifying the Vaxfectin:antigen ratio in the vaccine formulation.
MeSH terms
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Adjuvants, Immunologic / administration & dosage
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Adjuvants, Immunologic / pharmacology*
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Animals
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Antibodies, Viral / blood
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Antibody Formation
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Antigens, Viral / immunology
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Cations / immunology
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Cations / pharmacology
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Cells, Cultured
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Dose-Response Relationship, Immunologic
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Enzyme-Linked Immunosorbent Assay
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Female
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Hemagglutination Inhibition Tests
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Immunity, Cellular
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Immunity, Humoral
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Immunoglobulin G / blood
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Influenza Vaccines / administration & dosage
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Influenza Vaccines / immunology*
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Interferon-gamma / immunology
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Mice
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Mice, Inbred BALB C
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Mice, Inbred C57BL
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Orthomyxoviridae Infections / immunology
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Orthomyxoviridae Infections / prevention & control*
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Phosphatidylethanolamines / administration & dosage
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Phosphatidylethanolamines / immunology
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Phosphatidylethanolamines / pharmacology*
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Spleen / cytology
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Spleen / immunology
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Th1 Cells / immunology
Substances
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Adjuvants, Immunologic
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Antibodies, Viral
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Antigens, Viral
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Cations
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Immunoglobulin G
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Influenza Vaccines
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Phosphatidylethanolamines
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vaxfectin
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Interferon-gamma