Ferromagnetic levan composite: an affinity matrix to purify lectin

J Biomed Biotechnol. 2009:2009:179106. doi: 10.1155/2009/179106. Epub 2009 Jun 14.

Abstract

A simple and inexpensive procedure used magnetite and levan to synthesize a composite recovered by a magnetic field. Lectins from Canavalia ensiformis (Con A) and Cratylia mollis (Cramoll 1 and Cramoll 1, 4) did bind specifically to composite. The magnetic property of derivative favored washing out contaminating proteins and recovery of pure lectins with glucose elution. Cramoll 1 was purified by this affinity binding procedure in two steps instead of a previous three-step protocol with ammonium sulfate fractionation, affinity chromatography on Sephadex G-75, and ion exchange chromatography through a CM-cellulose column.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Canavalia / chemistry
  • Chromatography, Liquid
  • Concanavalin A / isolation & purification
  • Fabaceae / chemistry
  • Ferrosoferric Oxide / chemistry*
  • Fructans / chemistry*
  • Fructans / metabolism
  • Hemagglutination Inhibition Tests
  • Nuclear Magnetic Resonance, Biomolecular
  • Plant Lectins / isolation & purification*
  • Plant Lectins / metabolism
  • Zymomonas / chemistry

Substances

  • Fructans
  • Plant Lectins
  • Concanavalin A
  • levan
  • Ferrosoferric Oxide