Abstract
Anaplastic large cell lymphomas (ALCL) in children express anaplastic lymphoma kinase (ALK) fusion genes, most commonly NPM1-ALK. The distribution of X-ALK among 66 childhood ALCLs was analysed. One ALCL was ALK-negative. Reverse transcription polymerase chain reaction detected NPM1-ALK in 58 tumours, all showing nuclear and cytoplasmic ALK staining. The remaining seven ALCL stained for ALK in the cytoplasm only: two expressed TPM3-ALK, one ATIC-ALK, one MYH9-ALK; three no TPM3-, TFG-, ATIC-, CLTC- or MYH9-ALK. Almost 90% of paediatric ALK-positive ALCLs express NPM1-ALK. There was complete concordance between ALK staining pattern and the presence of a typical/variant ALK fusion partner.
MeSH terms
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Adolescent
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Anaplastic Lymphoma Kinase
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Child
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Child, Preschool
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Female
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Humans
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In Situ Hybridization, Fluorescence
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Infant
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Lymphoma, Large-Cell, Anaplastic / genetics*
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Lymphoma, Large-Cell, Anaplastic / metabolism
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Male
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Molecular Motor Proteins / metabolism
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Myosin Heavy Chains / metabolism
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Nucleophosmin
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Oncogene Proteins, Fusion / metabolism*
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Protein-Tyrosine Kinases / genetics*
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Protein-Tyrosine Kinases / metabolism
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Receptor Protein-Tyrosine Kinases
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Reverse Transcriptase Polymerase Chain Reaction
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Tropomyosin / metabolism
Substances
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ATIC-ALK fusion protein, human
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MYH9 protein, human
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Molecular Motor Proteins
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NPM1 protein, human
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Oncogene Proteins, Fusion
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TPM3 protein, human
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Tropomyosin
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oncoprotein CLTCL-ALK
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Nucleophosmin
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p80(NPM-ALK) protein
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ALK protein, human
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Anaplastic Lymphoma Kinase
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Protein-Tyrosine Kinases
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Receptor Protein-Tyrosine Kinases
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Myosin Heavy Chains