Controlled gene expression in the plant pathogen Cryphonectria parasitica by use of a copper-responsive element

Appl Environ Microbiol. 2009 Aug;75(16):5417-20. doi: 10.1128/AEM.00899-09. Epub 2009 Jun 19.

Abstract

We have developed a tool for controlled expression of heterologous or ectopic genes in the chestnut pathogen Cryphonectria parasitica using the promoter region from a putative copper-regulated transporter gene. In addition, we have found that expression control via this system is not affected by the virulence-attenuating hypovirus CHV1-EP713.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Ascomycota* / genetics
  • Ascomycota* / metabolism
  • Ascomycota* / pathogenicity
  • Ascomycota* / virology
  • Copper / pharmacology*
  • Fungal Proteins / genetics*
  • Fungal Proteins / metabolism
  • Gene Expression Regulation, Fungal / drug effects*
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism
  • Host-Pathogen Interactions
  • Molecular Sequence Data
  • Plant Diseases / virology*
  • Promoter Regions, Genetic
  • RNA Viruses / physiology
  • Sequence Analysis, DNA
  • Virulence

Substances

  • Fungal Proteins
  • enhanced green fluorescent protein
  • Green Fluorescent Proteins
  • Copper

Associated data

  • GENBANK/FJ627178