Abstract
We have developed a tool for controlled expression of heterologous or ectopic genes in the chestnut pathogen Cryphonectria parasitica using the promoter region from a putative copper-regulated transporter gene. In addition, we have found that expression control via this system is not affected by the virulence-attenuating hypovirus CHV1-EP713.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Ascomycota* / genetics
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Ascomycota* / metabolism
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Ascomycota* / pathogenicity
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Ascomycota* / virology
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Copper / pharmacology*
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Fungal Proteins / genetics*
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Fungal Proteins / metabolism
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Gene Expression Regulation, Fungal / drug effects*
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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Host-Pathogen Interactions
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Molecular Sequence Data
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Plant Diseases / virology*
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Promoter Regions, Genetic
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RNA Viruses / physiology
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Sequence Analysis, DNA
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Virulence
Substances
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Fungal Proteins
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enhanced green fluorescent protein
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Green Fluorescent Proteins
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Copper