Engineering and improvement of the efficiency of a chimeric [P450cam-RhFRed reductase domain] enzyme

Chem Commun (Camb). 2009 May 14:(18):2478-80. doi: 10.1039/b901716j. Epub 2009 Mar 24.

Abstract

A chimeric oxygenase, in which the P450cam domain was fused to the reductase host domains of a P450RhF from Rhodococcus sp. strain NCIMB 9784 was optimised to allow for a biotransformation at 30 mM substrate in 80% overall yield, with the linker region between P450 and FMN domain proving to be important for the effective biotransformation of (+)-camphor to 5-exo-hydroxycamphor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Camphor 5-Monooxygenase / chemistry*
  • Camphor 5-Monooxygenase / genetics*
  • Oxidoreductases / chemistry*
  • Oxidoreductases / genetics*
  • Protein Engineering / methods*
  • Protein Structure, Tertiary
  • Recombinant Fusion Proteins / chemistry*
  • Recombinant Fusion Proteins / genetics*
  • Rhodococcus / enzymology
  • Transformation, Genetic

Substances

  • Recombinant Fusion Proteins
  • Oxidoreductases
  • Camphor 5-Monooxygenase