7-ketocholesterol induces endoplasmic reticulum stress in HT-29 cells

Z Naturforsch C J Biosci. 2009 Mar-Apr;64(3-4):307-10. doi: 10.1515/znc-2009-3-425.

Abstract

7-Ketocholesterol (7-Kchol, oxidized cholesterol) is an important mediator of cell death in atherosclerosis mediated by up-regulated Nox 4 gene expression. In the current study using the human colon cancer HT-29 cell line, we have demonstrated that 7-Kchol promotes endoplasmic reticulum (ER) stress via gene up-regulation of ER chaperone and membrane kinases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line, Tumor
  • Colonic Neoplasms
  • DNA Primers
  • Endoplasmic Reticulum / drug effects
  • Endoplasmic Reticulum / physiology*
  • HT29 Cells / drug effects
  • HT29 Cells / physiology*
  • Humans
  • Ketocholesterols / pharmacology*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Signal Transduction / drug effects

Substances

  • DNA Primers
  • Ketocholesterols
  • 7-ketocholesterol