Abstract
Interaction of chiral Ru(II) salen complexes (S)-1 and (R)-1 with Calf Thymus DNA (CT-DNA) was studied by absorption spectroscopy, competitive binding study, viscosity measurements, CD measurements, thermal denaturation study and cleavage studies by agarose gel electrophoresis. The DNA binding affinity of (S)-1 (6.25 x 10(3)M(-1)) was found to be greater than (R)-1 (3.0 x 10(3)M(-1)). The antimicrobial studies of these complexes on five different gram (+)/(-) bacteria and three different fungal organisms showed selective inhibition of the growth of gram (+) bacteria and were not affective against gram (-) and fungal organisms. Further, the (S)-1 enantiomer inhibited the growth of organisms to a greater extent as compared to (R)-1 enantiomer.
MeSH terms
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Anti-Infective Agents / chemical synthesis
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Anti-Infective Agents / chemistry
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Anti-Infective Agents / metabolism
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Anti-Infective Agents / pharmacology
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Benzaldehydes / chemical synthesis*
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Benzaldehydes / chemistry*
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Benzaldehydes / metabolism*
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Benzaldehydes / pharmacology
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Binding, Competitive
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DNA / metabolism*
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DNA Breaks, Single-Stranded / drug effects
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Ethylenediamines / chemistry*
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Macromolecular Substances / chemical synthesis
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Macromolecular Substances / chemistry
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Macromolecular Substances / metabolism
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Macromolecular Substances / pharmacology
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Microbial Sensitivity Tests
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Models, Biological
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Nucleic Acid Denaturation
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Ruthenium / chemistry*
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Spectrum Analysis
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Stereoisomerism
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Temperature
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Viscosity
Substances
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Anti-Infective Agents
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Benzaldehydes
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Ethylenediamines
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Macromolecular Substances
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Ruthenium
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DNA
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calf thymus DNA
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disalicylaldehyde ethylenediamine