In this chapter, I expose the main properties and theoretical background of a somewhat out-of-fashion technique, multilocus enzyme electrophoresis (MLEE). I show that the remarkable properties of this marker-clear Mendelian inheritance, codominance, strong phylogenetic signal-are still valid, although of course more modern markers now are able to yield far more refined results. MLEE can still be used in many circumstances when a cheap and reliable marker is required. I summarize what have been the main contributions of MLEE to the study of parasites and other pathogens.